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. 2007 Jan 30;35(4):1119–1133. doi: 10.1093/nar/gkl1162

Figure 5.

Figure 5.

Absence of DSB formation in the GAL2 UAS region in cells co-expressing Gal4BD–spo11Y135F and Spo11-3FLAG. (A) DSB detection in the GAL2 UAS region (dashed bar in the right diagram). Strains and plugged DNA used in this experiment were the same as those for Figure 4. The plugged DNA was treated with NcoI and XbaI and probed for a GAL2 internal region (thin vertical bar in the right diagram). An asterisk and a filled triangle represent non-specific bands (EMP46 loci) and meiotic DSB bands introduced in the GAL2 UAS region, respectively. Note that no DSB band was detected in cells that co-express Gal4BD–spo11Y135F and Spo11-3FLAG. (B) Quantification of DSB in (A). DSB intensities (expressed as percentages relative to the total band intensity) are absolute and have not been corrected for background intensity. (C, D) DSB detection and quantification in cells co-expressing Gal4BD–Spo11 and spo11Y135F-3FLAG (YHS843). This strain is rad50S homozygous background. An asterisk in EMP46 loci presents non-specific bands. Quantification was performed as described in (B).

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