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. Author manuscript; available in PMC: 2007 Apr 11.
Published in final edited form as: J Mol Biol. 2006 Oct 6;365(4):1187–1200. doi: 10.1016/j.jmb.2006.09.092

Table 2.

Parameters for urea-induced unfolding of variants of the Notch ankyrin domain containing consensus ankyrin repeats at the C-terminus

ΔGºu H2O
 m-value
Cm
CD Fluorescence CD Fluorescence CD Fluorescence
Nank1-7* 7.74 ± 0.07a 7.64 ± 0.08 2.89 ± 0.04a 2.86± 0.02 2.68 ± 0.01a 2.67 ± 0.01
Nank1-5 3.21 ± 0.09 3.29 ± 0.03 1.78 ± 0.03 1.84 ± 0.01 1.80 ± 0.09 1.78 ± 0.02
Nank1-5C1 7.31 ± 0.17 7.50 ± 0.09 1.98 ± 0.10 2.05 ± 0.03 3.67 ± 0.04 3.65 ± 0.02
Nank1-5C2 13.02 ± 0.03 13.25 ± 0.4 2.28 ± 0.01 2.30 ± 0.07 5.70 ± 0.01 5.70 ± 0.01
Nank1-5C3 12.60 ± 0.12 13.66 ± 0.58 1.67 ± 0.03 1.83 ± 0.08 7.57 ± 0.14 7.48 ± 0.04

Unfolding free energies, m-values, and unfolding midpoints (Cm) are reported in kcal·mol−1, kcal·mol−1·M−1, and M, respectively. Uncertainties are standard errors on the mean of at least three independent measurements. Conditions: 25 mM Tris·HCl, 150 mM NaCl, pH 8.0, 20ºC.

a

Data for Nank1-7* are from reference 13.