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. Author manuscript; available in PMC: 2008 Jan 19.
Published in final edited form as: J Mol Biol. 2006 Oct 3;365(3):577–589. doi: 10.1016/j.jmb.2006.09.071

Figure 2.

Figure 2

Monitoring complex formation using native gel electrophoresis. Increasing concentrations of different length Notch1 polypeptides (0, 2 μ M, 3μ M, 5μ M, 7μ M, 9μ M, 10μ M, 12μ M, 15 μ M and 20μ M) were titrated into 10μ M BTD161–392. (A) Upper panel: 10μ M of RAMANKPPD was titrated with increasing concentrations of BTD161–392; lower panel; 10μ M of BTD161–392 was titrated with increasing concentrations of RAMANKPPD. (B) 10μ M of BTD161–392 was titrated with increasing concentrations of RAMANK. (C) 10μ M of BTD161–392 was titrated with increasing concentrations of RAMANKPPDWFP. (D) 10μ M of BTD161–392 was titrated with increasing concentrations of ANK.