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. Author manuscript; available in PMC: 2008 Jan 19.
Published in final edited form as: J Mol Biol. 2006 Oct 3;365(3):577–589. doi: 10.1016/j.jmb.2006.09.071

Figure 7.

Figure 7

Notch1 RAM peptides inhibit NICD:CSL association in cellular extracts. The effects of different RAM peptides on 6MT-NICD1:3XFLAG-RBPjκ (the mammalian CSL orthologues) interactions were assessed by immunodepletion. Shown are the amounts of 6MT-NICD1 remaining in the supernatants (analyzed by 9E10/MYC Western blots) after FLAG immunoprecipitation. (A) Lysates from cells expressing 6MT-NICD1 were combined with lysates from cells expressing 3XFlag-RBPjκ , and were immediately exposed to increasing concentrations (0.2, 1, 5 μ M) of different Notch1 RAM peptides (Lanes 1–12). (B) Lysates from cells co-expressing 6MT-NICD1 and 3XFlag-RBPjκ were pooled and similarly treated with different RAM peptides. As controls, immunodepletion assays were also performed without peptide (Lane 13–14) and with 6MT-NICD RAM expression vector (Lane 14).