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. Author manuscript; available in PMC: 2008 Jan 19.
Published in final edited form as: J Mol Biol. 2006 Oct 3;365(3):577–589. doi: 10.1016/j.jmb.2006.09.071

Table 1.

Calorimetric data for binding of Notch polypeptides to the BTD domain of CSL

BTD domain Of Ligand K (M−1) Kd (μM) ΔG° (kcal/mol) ΔH° (kcal/mol) TΔS° (kcal/mol)
BTD 161–349 RAMANKPPD 4.3( ± 2.5) x106 0.23 −9.2 ± 0.4 −9.0 ± 0.6 1.2 ± 0.5
BTD161–349 RAMANK 4.9( ± 0.5) x 106 0.20 −9.2 ± 0.2 −9.4 ± 0.4 −0.2 ± 0.6
BTD161–349 RAM 6.48( ± 2.3) x106 0.15 −9.4 ± 0.25 −10.9 ± 0.8 −1.5 ± 0.6
BTD161–349 N1RAM13 8.8( ± 1.4) x105 1.0 −8.2 ± 0.1 −16 ± 0.5 −7.8 ± 0.6
BTD161–349 N1RAM16 9.9( ± 0.45) x105 1.1 −8.2 ± 0.2 −8.4 ± 1.0 0.2 ± 0.7
BTD161–349 N1RAM22 1.5( ± 0.3) x106 0.8 −8.6 ± 0.1 −14.6 ± 0.8 −6 ± 0.8
BTD161–349 N2RAM23 2.0( ± 0.4) x 106 0.5 −8.7 ± 0.1 −9.4 ± 0.7 −0.7 ± 0.6
BTD161–349 N3RAM25 6.3( ± 0.1) x105 1.6 −8.0 ± 0.1 −12.4 ± 1.0 −4.4 ± 0.7
BTD161–349 N4RAM25 1.4 ( ± 0.7) X106 0.7 −8.5 ± 0.5 −11.2 ± 1.0 −2.7 ± 0.8

Reported values are the mean of at least three independent titrations. Uncertainties in K, ΔG° and ΔH° represent the standard deviations of multiple experiments. Uncertainty in TΔS° was calculated by standard error propagation.