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PP1 inhibition by A kinase-phosphorylated I-1 and by NCLK-phosphorylated I-1 and I-1(T35A). PP1 activity was assayed in the presence of indicated concentrations of the indicated inhibitor. After 30 min, aliquots were withdrawn and PP1 activity was analyzed. All of the assays were carried out at the same time under identical conditions.
