Table VI.

Candidate gene characteristics

*, Primers and restriction sites used for PCR markers are as follows: MDHAR1, GGAACCAAAGAGGAGTATGAGGC and CGACGTGTAAAAATCTTCCGTGGGG, PvuII site. MDHAR2, GCCATATGCACCATATGAACGTCC and CCCTTGTTCCTTGTACCAATCAGG, TaqI site. MDHAR3, GGGCAAGTTGAAGAGGAGAAGGG and GGAAAAGTGGCAACATCACCC, HinfI site. DHAR2, GGGCAAAGAACTGTCTTACAGCC and GCCACTTCCACAAGTCATTGGACC, size polymorphism. APX, CCTTACTGGCACCTGAGGTGCG and GCTCCACAAAGTATGAGTTGTC, SspI site. VTC2, GCTTATTACGTACAGTTGTATC and CCAGTTAGATAGACTTGGTTTAG, AluI site. **, Mapping of two independent clones gave the same profiles.

Gene	Function	TC No.	Clone No.	Tomato Library	Marker	Bin
GMP1	GDP-Man pyrophosphorylase	TC99113	cLEM8J23	Immature green	RFLP**	3-D
		TC99112	cLEG62O22	Fruit/breaker fruit
GMP2	GDP-Man pyrophosphorylase	TC110163	cLEM7F10	Immature green fruit	RFLP	9-E
GME1	GDP-Man epimerase	TC99842	cLEN10C18	Red ripe fruit	RFLP	1-G
GLD	l-Galactono-γ-lactone dehydrogenase	TC112703	cLHT24N6	S. habrochaites	RFLP**	10-E
		TC103695	cTOC18F20	Trichome/flower
AO	Ascorbate oxidase	TC99795	cLEC67D22	Tomato callus	RFLP**	4-E
MDHAR1	Monodehydroascorbate reductase	EST263177 TC singleton SGN-U155072	cLES18B10	Tomato leaf	PCR*	2-J
MDHAR2	Monodehydroascorbate reductase	TC126092	cTOD20A21	Tomato flower, anthesis	PCR*	8-G
MDHAR3	Monodehydroascorbate reductase	TC124435	cTOC25K2	Tomato flower, 8 mm, preanthesis buds	PCR*	9-D
DHAR1	Dehydroascorbate reductase	TC116433	cLEI16N22	Tomato germinating seed	RFLP	5-H
DHAR2	Dehydroascorbate reductase	TC117375	cLEG59I24	Tomato breaker fruit	PCR*	11-D
APX	Ascorbate peroxidase (putative peroxisomal)	TC161616	cTOE3O17	Tomato crown gall	PCR*	2-I
VTC2	Unknown protein	TC153912	cTOD24H13	Tomato flower, anthesis	PCR*	6-D
PGI	Phosphoglucose isomerase	TC98796	cLEB8K2	Tomato shoot	RFLP	12-D