Table III.
The incorrect processing of OsBADH2 transcripts in rice seedling and mature leaf
| Line | Tissue and Growth Condition | No. of Clones | Position and Size of Deletion | SDR | Nearly Identical Sequences |
|---|---|---|---|---|---|
| Nipponbare | Seedling, unstressed | 3 | e1. 42-e2. 102 (170)a | CGCCGG | CGtCGCCGGCG/CGCGCCGGgCG |
| Nipponbare | Seedling, drought | 5 | e1. 7-e2. 81 (184) | GGCC | GGCCaCG/GGCCgCG |
| Nipponbare | Seedling, drought | 1 | e1. 44-e2. 94 (160 bp) | GCG | GGCGaG/GcGCGcG |
| Nipponbare | Seedling, 0.25 m KCl, 24 h | 4 | e1. 6-e3. 26 (273 bp) | TGGC | GAGaTGGC/GAGcTGGC |
| Nipponbare | Seedling, 0.25 m KCl, 24 h | 3 | 5′ UTR −12-e2. 110 (231) | CCGTC | CCGTCgCG/CCGTCCG |
| Nipponbare | Seedling, 0.25 m KCl, 24 h | 2 | 5′ UTR −2-e2. 105 (216) | GGCG | CGGCG/CgGGCG |
| Nipponbare | Seedling, 0.25 m KCl, 24 h | 2 | e1. 41-e2. 108 (177) | CGCCG | GtCGCCGgC/GCGCCGtC |
| Nipponbare | Seedling, 0.25 m KCl, 24 h | 2 | e1. 42-e2. 102 (170) | CGCCGG | CGtCGCCGGCG/CGCGCCGGgCG |
| Nipponbare | Seedling, 0.5 m NaCl, 24 h | 2 | e1. 42-e2. 102 (170) | CGCCGG | CGtCGCCGGCG/CGCGCCGGgCG |
| Nipponbare | Seedling, 0.5 m NaCl, 24 h | 8 | e1. 2-e2. 85 (193)b 40-bp insertion from putative acyl-CoA oxidase | CGGCGAGA CTGGGC | CGGCGAGAtGGCCAC/CGGCGAGAgGGCCAC GaCTGGGCG/GcCTGGGCtG |
| 93-11 | Seedling, unstressed | 3 | e1. 53-e2. 55 (112) | GGCGCG | GtGGCGCG/GcGGCGCG |
| 93-11 | Seedling, 0.1 m NaCl, 72 h | 1 | e1. 55-e2. 123 (178) | CC | CCcCCGCGC/CCtCCGCGC |
| 2 | e2 (143 bp)c | ||||
| Minghui 63 | Seedling, unstressed | 1 | e1. 42-e2. 102 (170) | CGCCGG | CGtCGCCGGCG/CGCGCCGGgCG |
| Minghui 63 | Seedling, 0.1 m NaCl, 72 h | 1 | e1. 42-e2. 102 (170) | CGCCGG | CGtCGCCGGCG/CGCGCCGGgCG |
| Suhui 527 | Seedling, unstressed | 1 | e1. 21-e2. 96 (185)d 59-bp insertion from putative peptidylprolyl isomerase | GCA C | GCAgCGGCAG/GCAcCGGCgAG CgCCGgG/CaCCGcG |
| Suhui 527 | Seedling, 0.1 m NaCl, 72 h | 1 | 5′ UTR −11-e2. 112 (232) | CG | CCGTCG/CCGTcCG |
| Chuanxiang 29B | Seedling, unstressed | 1 | e1. 41-e2. 108 (177) | CGCCG | GtCGCCGgC/GCGCCGtC |
| Chuanxiang 29B | Seedling, 0.1 m NaCl, 72 h | 1 | e1. 23-e5. 63 (523) | CAGC | CAGCgG/CAGCtG |
| Zhonghua 9 | Seedling, unstressed | 1 | e1. 21-e2. 131 (220) | CGCA | TCCCGCA/TCCgCGCA |
| Zhonghua 9 | Seedling, 0.1 m NaCl, 72 h | 1 | 5′ UTR −2-e2. 101 (212) | CG | CGgCGaG/CGcCGgG |
| Yuanlixiangjing | Seedling, unstressed | 1 | e1. 52-e2. 106 (164) | GGCGC | GtGGCGCG/GGGCGCcG |
| Yuanlixiangjing | Seedling, 0.1 m NaCl, 72 h | 1 | 5′ UTR −8-e2. 85 (202) | CGCGA | GtCGCGAtC/GcCGCGAC |
| Leab | Seedling, unstressed | 1 | e1. 55-e2. 107 (162) | GCGCC | GGcGCGCC/GGGCGCC |
| Leab | Seedling, 0.1 m NaCl, 72 h | 1 | 5′ UTR −8-e2. 85 (202) | CGCGA | GtCGCGAtC/GcCGCGAC |
| Newbonnet | Seedling, unstressed | 1 | 5′ UTR −10-e2. 106 (225) | CGC | GtCGCG/GCGCcG |
| Newbonnet | Seedling, 0.1 m NaCl, 72 h | 1 | e1. 18-e2. 140 (232) | CC | |
| Tebonnet | Seedling, unstressed | 1 | e1. 24-e2. 98 (184) | GCG | CGCaGCG/CGCGCG |
| Tebonnet | Seedling, 0.1 m NaCl, 72 h | 1 | e1. 24-e2. 84 (170) | GCG | GCaGCGgC/GCcGCGaC |
| Nipponbare | Mature leaf, field | 2 | 5′ UTR −3-e2. 115 (227) | GGC | CGGCgA/CgGGCcA |
| 93-11 | Mature leaf, field | 1 | 5′ UTR −10-e2. 130 (249) | CGC | CCGtCGCgATCG/CCGCGCaATCG |
| Chuanxiang 29B | Mature leaf, field | 2 | 5′ UTR −3-e2. 99 (211) | GC | CGGCG/CGcGCcG |
a
e1. 42-e2. 102 (170) denotes that exon materials of OsBADH2 from the 42nd base of exon 1 to the 102nd of exon 2 were excised; 170 nucleotides in total were deleted.
b
In these cDNAs, the deletions were replaced by a fragment derived from putative acyl-CoA oxidase (accession no. XM_476282).
c
Selective deletion of entire exon 2.
d
In this cDNA, the deletion was replaced by a fragment derived from putative peptidylprolyl isomerase (accession no. XM_463914).