Figure 7.

Expression of HKT1;5 in wheat analyzed using reverse-transcriptase PCR. A, Expression of TmHKT1;5-A. A product of the expected size (442 bp) was observed for the T. monococcum and Line 149 root samples. B, Expression of TaHKT1;5-D. A product of the expected size (147 bp) was observed for the CS root sample. Mon = T. monococcum; L149 = Line 149; Tam = Tamaroi; 0.86 = CS deletion line missing the distal 14% of chromosome 4DL; n = no template control; R = root tissue; L = leaf tissue. Plants were grown in hydroponic solution for 2 weeks before addition of 50 mm NaCl, tissue was harvested 48 h after addition of NaCl. Proton pump was used as a control.