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Quantification of PME activity in AtPMEI-1, AtPMEI-2, and wild-type plants with radial gel diffusion assay
PME activities (%) were determined from leaves of AtPMEI-1 and AtPMEI-2 transgenic lines relative to wild type. Data represent average ± se of five biological replicates. The PME activity of the wild-type leaves (0.013 units) was set to 100%.
| Line | PME Activity |
|---|---|
| % | |
| Wild type | 100 |
| 1-43 | 85.9 ± 0.7 |
| 1-1 | 61.6 ± 2.3 |
| 1-5 | 46.7 ± 1.1 |
| 2-15 | 83.8 ± 0.2 |
| 2-7 | 52.4 ± 0.3 |
| 2-9 | 44.7 ± 0.8 |
