Table III.
Nominal masses, composition, and significance of ions generated by MALDI-TOF MS of the various AtPMEI transgenic lines
| Nominal Massa | Compositionb | 1-1c | 1-5c | 2-7c | 2-9c |
|---|---|---|---|---|---|
| 759 | G4(1,0) | ||||
| 801 | G4(1,1) | * | * | ||
| 935 | G5(1,0) | ||||
| 949 | G5(2,0) | * | * | ||
| 977 | G5(4,0) | * | * | ||
| 991 | G5(5,0) | * | * | * | * |
| 1033 | G5(1,2) | ||||
| 1153 | G6(4,0) | ||||
| 1167 | G6(5,0) | ||||
| 1386 | G7(4,1) |
a
Nominal mass (M+Na+)+ of pectic oligosaccharides released by endoPG/PME digestion of Arabidopsis etiolated seedling cell wall material.
b
Gn(m,a) fragment; “Gn” denotes the number of galacturonic acid residues in the backbone; and “m” and “a” the number of methylesterified and acetylated residues, respectively.
c
AtPMEI-1 or AtPMEI-2 transformed lines. * and bold, Ion areas significantly different compared with wild type by t test (P = 0.01; data were obtained from 30 independent samples for wild type and 20 independent samples for each transformed line; 10 seedlings were used for each sample).