Figure 5.
The effect of nigericin and DTT on NPQ formation. The dynamics of the transient NPQ were determined in wild-type and NPQ-mutant plants in the presence of 50 μm nigericin (A) and 5 mm DTT (B). Uptake of inhibitors was performed by vacuum infiltration. NPQ formation was induced by illumination of the leaves at a light intensity of 100 μmol photons m−2 s−1. All other conditions are as described in the legend to Figure 1. Mean values (±sd) of three to four independent experiments are shown. For clarity, sd is shown for only wild-type plants in A but was in the similar range for all other genotypes. In B, sd for npq1 plants is not shown but was in the same range as shown for wild-type plants. It should be noted that the scale of the y axis is spread by a factor of 2 in comparison with all other figures.