Figure 7.

Content and redox states of 2-Cys Prx in 3-week-old rimb mutants. Left: The total 2-Cys Prx protein amount was determined in DTT-treated plant samples by western-blot analysis with 2-Cys Prx antibody and quantification of the band intensities. Middle: Intermolecular disulfide bond formation was analyzed in protein samples extracted immediately in SDS-containing buffer by 20% SDS-PAGE, western-blot analysis with 2-Cys Prx-specific antibodies, and quantification of band intensities. Right: The pI of 2-Cys Prx was determined by 2-D PAGE with immobilized pH gradients (pH 4–7) and western-blot analysis with 2-Cys Prx-specific antibodies.