Figure 9.

Functional expression of ZmYS1 improves growth of Arabidopsis plants under Cd stress in the presence of Fe phytosiderophore. A, RNA gel-blot analysis of Arabidopsis root RNA extracted from wild-type or homozygous T3 plants transformed with a CaMV35S:ZmYS1 construct. ZmYS1 expression was detected in hydroponically grown, Fe-sufficient plants. Ethidium bromide-stained gel blots are shown as a loading control. B, Growth phenotype of plants grown on modified 0.5 Murashige and Skoog agar plates supplemented with 20 μm Fe(III)-EDTA or Fe(III)-DMA. Plants in bottom section were grown in the presence of 15 μm CdCl₂. Plates of representative plants were photographed 10 d after transfer of seedlings to the treatments. Bar = 3 cm. C, Quantitative analysis of shoot growth after plant cultivation as described in B with different Fe-binding forms and Cd concentrations. D, Chlorophyll concentrations in shoots of plants as grown in B. Significant differences at P < 0.05 as determined by ANOVA followed by Tukey test are indicated by different letters (n = 7). Presented data are representative for three independent experiments.