Figure 2.

Dose-response effects of DF on Arabidopsis seedling growth and adenine nucleotide pools. Five-day-old seedlings were transferred onto Murashige and Skoog salt (0.5×) + 1% Suc agar supplemented with increasing concentrations of DF (0–450 nm) and grown for 9 d under long-day conditions (16 h light/8 h dark). Dose-dependent decrease in seedling growth (top left) is associated with an increase in total adenine nucleotides (TAN; top right), which affects all phosphorylated species: ATP, bottom left; ADP, bottom middle; and AMP, bottom right. Data expressed as the mean ± sd (n = 6). *, P < 0.05 when compared to control in an unpaired two-tailed t test. All metabolites are expressed as nmol/g wet weight.