Figure 4.

Molecular characterization and phenotypes of OsMYB3R-2 transgenic Arabidopsis plants. A, Southern-blot assay for Arabidopsis transgenic plants. Genomic DNA isolated from the wild-type (WT) or transformed plants digested with EcoRI (E) or HindIII (H). The blot was hybridized with the open reading frame of the GUS gene labeled with α-³²P-dCTP and α-³²P-dATP as described in “Materials and Methods.” B, Expression of independent transgenic plant lines of Arabidopsis by RNA gel-blot analysis. Each lane was loaded with 10 μg total RNA isolated from 3-week-old seedlings of transgenic Arabidopsis. The RNA blot was hybridized with a ³²P-labeled OsMYB3R-2 cDNA probe. Ethidium bromide-stained rRNA was used as a RNA-loading control. C, The phenotypes of the T3 generation of independent lines of overexpressed OsMYB3R-2 transgenic Arabidopsis. Wild-type and OsMYB3R-2-overexpressed lines after 3 weeks growth at 22°C. [See online article for color version of this figure.]