Figure 6.

Germination potential of cts-1 rgl2-1 seeds and expression of GA biosynthesis genes in wild-type and cts-1 seeds. A, Germination potential after 7 d is shown for freshly harvested and S/AR seed lots assayed on WA media either with or without exogenous PAC (10 μm). Data represent mean and sd. B, Quantitative RT-PCR analysis of genes encoding AtGA3ox1 and 2 in Ler and cts-1 seeds. RNA was obtained from Ler D and AR and cts-1 S and F seeds imbibed on WA over 48 h. AGI numbers and function of encoded proteins are indicated for each transcript.