Figure 8.

Expression of genes of the flavonoid biosynthesis pathway in wild-type and cts-1 seeds. A, Schematic representation of flavonoid biosynthesis (redrawn from Peer et al., 2001). Enzymes are shown with associated position in the Ler AR > cts-1 S seed gene set and fold differential up-regulation in AR seeds (see Supplemental Table S1). 4CL, 4-Coumarate-CoA ligase; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone-3-hydroxylase; F3′H, flavonoid 3′-hydroxylase; FLS, flavonol synthase; TT, transparent testa; PAP2, PRODUCTION OF ANTHOCYANIN PIGMENT 2; EGL3, ENHANCER OF GLABRA 3. B, Quantitative RT-PCR analysis of gene expression in wild-type Ler and cts-1 S seeds imbibed on WA.