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Effects of mutations in the C terminus of AtAMT1;2 on NH4+ transport. A, Growth of AMT-deficient yeast transformed with AtAMT1;2 wild type and mutants on 3 mm ammonium as sole nitrogen source (top). The same growth assay for the mutants in an AtAMT1;2-GFP fusion protein (bottom) is also shown. B, Fluorescence pattern of GFP-tagged AtAMT1;2 wild type and mutants in yeast (left) and bright-field images (right). Scale bars = 4 μm. [See online article for color version of this figure.]
