Figure 3.

Coexpression of ERD2-GFP with YFP-Sec24 increases the fluorescence and number of ERES. A to C, YFP-Sec24 labels the cytosol (arrowhead) and punctate structures (ERES, arrow) when expressed alone. Note that there is no signal in the GFP channel, excluding the possibility that colocalization of the signals is due to imaging cross talk (Brandizzi et al., 2002b). Coexpression of ERD2-GFP (D) with YFP-Sec24 (E) results in an apparent increase in the fluorescence intensity of the YFP-Sec24 at ERES (E). F, Merged image of D and E. Coexpression of the soluble marker secGFP (G) with YFP-Sec24 (H) does not appear to affect the number or fluorescence of ERES (I, merge). Bars = 5 μm.