Seed germination in T-DNA insertion mutants of GCR1, PRN1, NF-Y- B9 (LEC1), NF-Y-B6 (LEC1 LIKE), and NF-Y-C4 are hypersensitive to ABA. Matched seed lots of wild-type Col and T-DNA insertions in genes: GCR1 (SALK_027808), PRN1 (SALK_006939), NF-Y-B9 (LEC1; SALK_000450), NF-Y-B6 (LEC1 LIKE; SALK_118236), NF-Y-B2 (SALK_025666), NF-Y-A5 (SALK_006559), NF-Y-A4 (SALK_003337), NF-Y-C1 (SALK_086334), and NF-Y-C4 (SALK_032163) were planted on media supplemented with (+) 600 nm ABA or without (−) ABA, stratified, then placed at 22°C in continuous white light as described (Lapik and Kaufman, 2003). Germinating seeds were counted every day after planting as described (Lapik and Kaufman, 2003). Representative photographs of germination and early seedling development taken 7 d after stratification are shown.