Figure 2.

SDH1-1/sdh1-1 plants contain a unique complex T-DNA insertion. Southern-blot analysis was performed to characterize the insertion. Total DNA (6 μg) from wt (w) or mutant (m) plants was digested with PstI and hybridized sequentially with probes directed to the T-DNA (GUS probe), the SDH1-1 upstream region (probe 1), and the SDH1-1 downstream region (probe 2). The presence of a new PstI restriction site (P) was inferred from the hybridization results and is shown in gray, in a sequence of unknown origin.