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. 2007 Apr;143(4):1534–1546. doi: 10.1104/pp.106.095158

Figure 5.

Figure 5.

Pollen development in SDH1-1/sdh1-1 mutant plants. Anther sections were stained with Toluidine blue and photographed by bright-field microscopy. Abnormal pollen grains are indicated by arrowheads. Magnification is the same in all micrographs (Bars = 20 μm). GN, Generative cell nucleus; N, nucleus; Tp, tapetum; V, vacuole; VN, vegetative cell nucleus. A, Anther from the wild type at the vacuolated microspore stage. B, Anther from the SDH1-1/sdh1-1 mutant, at the vacuolated microspore stage. All microspores are indistinguishable from those of wild-type anthers. C, Anther from the SDH1-1/sdh1-1 mutant after pollen mitosis I, showing normal pollen grains with two nuclei, and abnormal pollen grains (arrowheads) not entering mitosis. D, Anther from the SDH1-1/sdh1-1 mutant after pollen mitosis II, showing normal pollen grains with a dense cytoplasm, and abnormal pollen grains (arrowheads) containing cell debris. E, Anther just before dehiscence, containing mature pollen grains and collapsed pollen grains devoid of cytoplasm (arrowheads).