Table I.
Kinetic parameters of SalR of P. bracteatum for the forward and reverse reaction and the cofactors
| Substratesa
|
Cofactorsbc
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Km | Ki | Vmax | Vopt | Kcat | KcatKm−1 | Km | Vmax | Kcat | KcatKm−1 | |
| μm | μm | nkat mg−1 | nkat mg−1 | s−1 | s−1m−1 103 | μm | nkat mg−1 | s−1 | s−1m−1 103 | |
| Salutaridine | 7.9 ± 2.8 | 140 ± 57 | 93.8 ± 15 | 63.2 ± 8 | 2.3 ± 0.3 | 291 | 3.5 ± 0.8 | 57.5 ± 2.3 | 2.1 ± 0.1 | 600 |
| Salutaridinol | 1.5 ± 0.2 | – | 588 ± 16 | – | 21.6 ± 0.6 | 14,400 | 7.0 ± 0.9 | 598 ± 17 | 21.9 ± 0.6 | 3,128 |
a
NADPH as cofactor for salutaridine, NADP for salutaridinol, 250 μm each.
b
NADPH as cofactor for salutaridine; salutaridine concentration 10 μm.
c
NADP as cofactor for salutaridinol; salutaridinol concentration 50 μm.