Table II.
Kinetic parameters with respect to the cofactors of mutated variants of SalR designed for the investigations on cofactor preference
| Variant | Cofactor | Km | Vmax | Kcat | KcatKm−1 |
|---|---|---|---|---|---|
| μm | nkat mg−1 | s−1 | s−1m−1 103 | ||
| Wild type | NADPH | 3.5 ± 0.8a | 57.3 ± 2.3 | 2.1 ± 0.093 | 600 |
| NADH | 1,190 ± 43b | 102 ± 1.1 | 3.7 ± 0.04 | 3.1 | |
| R48K | NADPH | 9.4 ± 1.2c | 60.6 ± 1.7 | 2.2 ± 0.06 | 234 |
| NADH | 645 ± 72b | 77 ± 2.4 | 2.8 ± 0.09 | 4.3 | |
| R48E | NADPH | 104 ± 10d | 95 ± 3.3 | 3.5 ± 0.12 | 33.6 |
| NADH | 884 ± 141e | 45.8 ± 2.4 | 1.7 ± 0.08 | 1.9 | |
| R44E | NADPH | 187 ± 29d | 18.7 ± 1.02 | 0.7 ± 0.03 | 3.7 |
| NADH | 1,257 ± 91f | 159 ± 3.7 | 5.8 ± 0.14 | 4.6 |
a
Salutaridine concentration 10 μm.
b
Salutaridine concentration 100 μm.
c
Salutaridine concentration 20 μm.
d
Salutaridine concentration 50 μm.
e
Salutaridine concentration 150 μm.
f
Salutaridine concentration 250 μm.