Figure 4.

Involvement of HIF-1α in the biology of MiaPaCa2 cells in the presence of insulin. Cells were cultured as described in Figure 3, but insulin was added to the test media in concentrations of 0.1 to 1000 nmol/L. After incubation, test media and cell homogenates were examined as described in Figure 3. The present figure shows the effects of insulin on glucose consumption (ie, the difference between original and measured glucose concentrations, A), lactate production (B), VEGF secretion (C), and thymidine uptake (D) in each cell line, using the values in the absence of insulin as 100%. Results are derived from six individual experiments. In each experiment, each condition was tested in three wells of cells. *P < 0.05 and **P < 0.01.