Figure 7.

Transfection of PPARγ into SUP-M2 cells improves cell survival and suppresses ROS accumulation. A: PPARγ or pcDNA3 vector was stably transfected into SUP-M2 cells. Western blot shows the presence or absence of the PPARγ protein expression in stable cell lines containing PPARγ expression vector or pcDNA3 vector. B: Cell survival in serum-deprived SUP-M2 cells with or without PPARγ. Rosiglitazone (2 μmol/L) was added at the time of serum withdrawal. Individual stable clones are shown on the left and pools of stably transfected cells are shown on the right. C: ROS levels in serum-deprived SUP-M2 cells with or without PPARγ. Rosiglitazone (2 μmol/L) was added at the time of serum withdrawal. Individual stable clones are shown on the left and pools of stably transfected cells are shown on the right.