Figure 10.

Production of IFN-β and LDH release by transfected HFHs. Cultures were transfected with WT HCV RNA and maintained for 64 days. Medium was collected at the days indicated in the panels, and 100 μl was used for the determination of IFN-β (A) by a specific ELISA (human IFN-β; PBL Biomedical Laboratories, Piscataway, NJ) and LDH (B, stippled bars) using the SYNCHRON LX system (Beckman Coulter). Media from cultures of cells transfected with NS5B mutant RNA are also shown in B (black bars). For reference, B also includes LDH measurements in media from untransfected cultures (Untrx) and cultures treated for 2 days with 10 mmol/L acetaminophen (APAP). All samples were tested in duplicate.