Figure 5.

Infection of naïve HFHs with culture media from transfected cells. Naïve HFHs were infected by medium of transfected HFHs obtained at 16 (A) or 37 (B) days after transfection. After 2-day co-cultivation (see Materials and Methods), the inserts containing transfected HFHs were removed, and the naïve HFHs were cultured in fresh medium. Viral levels in the medium collected at the end of the 2-day co-cultivation period is labeled as CC in A and B. Media were then collected for 40 days (A) or 20 days (B) for HCV quantification.