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Histological analysis of heterotopic bone formation stimulated by BMP2. a: Day 1, cells morphologically similar to brown adipocytes (arrows) detected between muscle fibers. b: Day 3 or 4, immature vessels (arrows) leaking red blood cells (inset, VWF staining). c: Day 5 or 6, cartilage matrix with embedded chondrocytes [arrows; inset, S-100 staining; muscle (M)]; d: Day 7, bone (B). Original magnifications: ×20.5 (a, c); ×10.5 (b, d).
