Figure 1.

PKC regulates p60^src activity in HCs; active p60^src and PKC are required for tyrosine phosphorylation of Vav. Cells (10⁷/ml) were incubated for 60 minutes with dimethylsulfoxide (control) or with 10 μmol/L Ro32-0432, safingol, PP1, or SU6656. A shows p60^src in lysates of 10⁶ cells Western blotted for activation-dependent Tyr-416 phosphorylation (top panel). The center and bottom panels show levels of p60^src protein and β-actin as loading controls. In B, p60^src was inhibited by treatment of HCs with PP1 or SU6656 and in C, by safingol or Ro32-0432. Then proteins in cell lysates (10⁶ cells) were subjected to SDS-PAGE and immunoblotted with anti-phospho-Vav antibody (Tyr-174). Total Vav and β-actin were used as loading controls.