Figure 2.
Rac1 and Cdc42 are constitutively active and control the morphological appearance of HCs. A–D: HCs (107 cells/ml) were cultured on polyHEMA-coated plates in the absence of serum and lysed at the indicated times. A: Proteins isolated by Rac1-pulldown were immunoblotted with anti-Rac1 or anti-Cdc42 antibodies and compared with the total Rac1 or Cdc42 contained in cell lysates. B: Rac1 pulldown of HCs treated with 10 μmol/L PP1 for 1 hour. C: Rac1 pulldown of HCs treated with 10 μmol/L Ro32-0432 for 1 hour. D: Rac1 pulldown of HCs treated with 20 ng/ml toxin B for 3 hours. In B–D, similar results were obtained using cells of another HCL case. E: Cytospins of control HCs or HCs treated for 3 hours with 20 ng/ml toxin B or for 1 hour with 10 μmol/L Ro32-0432 or with 10 μmol/L PP1. HCs were stained with May-Grunwald-Giemsa and examined with light microscopy.