Table 1.
Quantification of Synaptic AChR and Rapsyn Relative to VAChT
| Animal | Antibody titer (nmol/L)* | Untreated muscle
|
Rapsyn-treated muscle
|
||
|---|---|---|---|---|---|
| AChR† | Rapsyn† | AChR† | Rapsyn† | ||
| 1 | 20.0 | 0.84 ± 0.48 | 0.51 ± 0.04 | 0.63 ± 0.19 | 0.44 ± 0.10 |
| (n = 397) | (n = 470) | ||||
| 2 | 29.6 | 0.56 ± 0.04 | 0.37 ± 0.03 | 1.10 ± 0.21 | 0.60 ± 0.2 |
| (n = 317) | (n = 301) | ||||
| 3 | 32.8 | 0.71 ± 0.16 | 0.41 ± 0.09 | 0.69 ± 0.01 | 0.51 ± 0.43 |
| (n = 325) | (n = 315) | ||||
| 4 | 68.1 | 0.46 ± 0.12 | 0.37 ± 0.02 | 0.53 ± 0.26 | 0.60 ± 0.45 |
| (n = 343) | (n = 436) | ||||
| 5 | 154.0 | 0.45 ± 0.06 | 0.20 ± 0.02 | 0.51 ± 0.13 | 0.28 ± 0.08 |
| (n = 281) | (n = 394) | ||||
*
The antibody titer is represented as the equivalent of α-BT binding sites of precipitated AChR.
†
The staining intensity of AChR and rapsyn was measured relative to presynaptic staining of VAChT. The results are normalized relative to average AChR and rapsyn levels of endplates in untreated control muscles. The values are the averages of two independent experiments (±SD) measuring protein levels in representative areas of the muscles. The number in parentheses is the total amount of endplates analyzed in both experiments.