TIMP-1 detection in steatotic OLTs. Top panel illustrates TIMP-1 expression at mRNA level (A) in steatotic naïve livers (lane 1), and OLTs at day 1 (lanes 2 and 3), day 3 (lanes 4 and 5), and day 7 (lanes 6 and 7) after transplantation. Control livers (lanes 2, 4, and 6) showed higher levels of TIMP-1 mRNA expression in particular at days 1 and 7 post-OLT (lanes 2 and 6) compared with CS1-treated OLTs (lanes 3, 4, and 5). However, at protein level (B, bottom panel), TIMP-1 was virtually undetectable in steatotic livers with the exception of day 7 control OLTs (lane 6), which expressed relatively high levels of TIMP-1 (n = 4/group).