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. 2007 Feb;170(2):567–577. doi: 10.2353/ajpath.2007.060456

Figure 8.

Figure 8

Gelatinase detection in RAW 264.7 macrophage-like cells. A: Representative zymogram of RAW 264.7 cells. RAW 264.7 cells cultured on FN-coated plates (lane 4) expressed increased levels of MMP-9 mRNA compared with the respective control cells cultured in polylysine-coated plates (lane 3). In contrast, MMP-9 expression was significantly depressed in RAW cells cultured in FN-coated plates pretreated with anti-FN Ab (lane 5) or RAW cells incubated with an anti-α4 Ab (lane 6) before being plated on FN-coated plates. B: Densitometric ratios for MMP-9/β-actin mRNA were significantly increased in cells cultured on FN compared with cells cultured on polylysine, cells cultured on FN-coated plates pretreated with anti-FN Ab, and cells incubated with an anti-α4 Ab prior being plated on FN-coated plates (n = 4/group, *P < 0.05).