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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2006 Dec 8;1768(3):669–677. doi: 10.1016/j.bbamem.2006.12.004

Figure 2.

Figure 2

Effect of freeze-thawing and freeze-drying on the generation of reactive oxygen species (ROS). Levels of ROS assessed by monitoring the fluorescence intensity of proxyl fluorescamine at 385 nm following freeze-thawing (FT) and freeze-drying (FD) were assessed by using a fluorogenic spin trap probe, proxyl fluorescamine, that was added to the formulations prior to freezing or freeze-drying as described in Methods section. (*) Significant different sample with p < 0.0001. Reported values are represented as the mean ± 1 S.D. of triplicate determinations.