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Effect of increasing amounts of Cu2+ on the stability of DOTAP:DOPE/DNA complexes formulated in trehalose during acute lyophilization, in terms of (A) ROS content and transfection rates, or (B) DNA integrity. Percent of DNA is shown as supercoil content (SC), open circle (OC) and linear form (L) remaining in samples after acute lyophilization. The results are expressed as mean values ± 1 S.D. of measurements on triplicate samples.
