Immunoblot analysis of Crry and CD59 in normal rat aqueous humor. Aqueous humor (lanes 1, 3, and 5) was subjected to SDS-PAGE under nonreducing conditions. Solubilized preparation of normal rat splenocytes (lanes 2, 4, and 6) was used as the positive control. Blots were probed with mAb to rat Crry (A), rat CD59 (B), and MOPC-21 (C). Molecular masses, right.