Fig. 2.

Inhibition of MHV-induced oligodendrocyte apoptosis by treatment with a neutralizing antibody against the MHV spike protein. The experiments were carried out essentially the same as described in the legend of Fig. 1, except the initial treatment with the antibody as noted below. Cells were mock-infected or infected with live- or UV-inactivated MHV at 4 °C for 1 h. Virus-bound cells were washed with cold PBS twice and the neutralizing monoclonal antibody specific to the spike protein of MHV, termed J2.6, or IgG2b (as isotype control) was added to the culture and incubated at 4 °C for another hour. The culture was then incubated at 37 °C for 1 h to allow virus entry. Virus titers were determined only for live virus-infected culture at 24 h p.i. and were expressed as means ± SD from three independent experiments (A). Apoptosis was analyzed with PI staining at 48 h p.i. (B) or by detecting annexin V binding at 12 h p.i. (C) as described in the legend of Fig. 1.