TABLE 1. Pairwise comparison of immune response and protein expression data: summary of statistical data analysis.

The magnitude of the Gag-specific T cell response in vivo, as measured by tetramer staining or intracellular cytokine staining (ICS; data from Figure 6), was compared in a pairwise fashion with normalized data values for amplicon-directed Gag expression in cultured cells and tissue samples (data from Figure 2). The cells listed correspond to primary murine bone marrow-derived dendritic cells (DC) and to murine cell lines of fibroblast (3T3), keratinocyte (XB2) and epithelial (EMT6) lineage. A correlation coefficient (Pearson r value) was generated for each pairwise data comparison (using GraphPad PRISM), and is shown above. The corresponding P value is also shown (based on a two-tailed test). The data show that the magnitude of the in vivo T cell response was significantly correlated with the level of Gag antigen production both in vitro (in cultured cell lines and primary DC) and in vivo (in biopsied tissue samples).

Immune response parameter	Protein (Gag) production parameter	Correlation coefficient (Pearson r)	P value (two-tailed)
CD8+ Tetramer+ cells	DC (in vitro)	0.932	0.007
	3T3 (in vitro)	0.763	0.028
	XB2 (in vitro)	0.842	0.009
	EMT6 (in vitro)	0.882	0.004
	Tissue site (in vivo)	0.791	0.019
CD8+ IFNγ+ cells (ICS)	DC (in vitro)	0.982	<0.0001
	3T3 (in vitro)	0.810	0.015
	XB2 (in vitro)	0.797	0.018
	EMT6 (in vitro)	0.860	0.006
	Tissue site (in vivo)	0.797	0.018
CD4+ IL2+ cells (ICS)	DC (in vitro)	0.961	0.0001
	3T3 (in vitro)	0.880	0.004
	XB2 (in vitro)	0.861	0.006
	EMT6 (in vitro)	0.911	0.002
	Tissue site (in vivo)	0.884	0.004