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. 2007 Mar 30;7:20. doi: 10.1186/1472-6807-7-20

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Copyright © 2007 Sgraja et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The over-expression of TbMK and LmMK in procyclic-form T.brucei. TbMK and LmMK were cloned into the pLew82 vector and transformed into procyclic T. brucei to express ectopic C-terminal HA-tagged proteins under tetracycline control. Mid-log procyclic cells over-expressing TbMK-HA (lane1), LmMK-HA (lane 2) and wild type (lane 3) were washed, lysed and run on an SDS/10 % polyacrylamide gel and transferred to an ECL-Nylon membrane by Western blotting and detected with anti-HA antibodies. The expected molecular weight of both TbMK-HA and LmMK-HA is 36.9 kDa.