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. 2007 Apr 13;3(4):e54. doi: 10.1371/journal.ppat.0030054

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© 2007 Brandt et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Opposing arrows mark primer binding sites for the quantitative real-time PCR. Predicted secondary structures for wild-type (wt RRE) and mutated RRE are shown, with the grey stem loop representing the MS2-derived RNA motif. (A) HIV-1 vector constructs, (B) SIV vector constructs.

Blas-EGFP, EGFP cDNA fused in frame to the Blasticidin resistance gene; CMV, human cytomegalovirus immediate early promoter; EGFP, gene for the enhanced green fluorescent protein; MLV, murine leukemia virus promoter; R, direct repeat sequence; SA, splice acceptor; SD, splice donor; U3, 3′ unique sequence; U5, 5′ unique sequence.