Figure 3. DSB Repair Frequencies for lig4 and ku70 and Their Controls.

Experiment numbers, frequency estimates, and standard errors are from Table 1 except that HR-h frequencies were computed by first summing the long- with short-tract HR-h frequencies for each individual test male, then computing averages and standard errors. Significance values were computed as described in Materials and Methods. All comparisons are between a mutant genotype and its corresponding control. That is, experiments 2 and 3 are compared with experiment 1; experiments 11, 14, and 15 with 10, etc. Experiments 25 and 26, the heterozygous controls for ku70, were pooled since they were not significantly different from each other. NHEJΔ was not measured in experiment 10, cross 2 and the NHEJ value for that control includes NHEJΔ events. However, since the NHEJΔ frequency in other controls (C) were less than 0.5%, this difference is not expected to affect the use of experiment 10 as the NHEJ control for experiments 11, 14, and 15 in (A). Shown are: (A) NHEJ cross 2; (B) NHEJ cross 1; (C) NHEJΔ cross 2; (D) NHEJΔ cross 1; (E) SSA cross 2; (F) SSA cross 1; (G) HR-h cross 2 with long- and short-tract events pooled.