Figure 3.

Interference of protease-resistant ³⁵S-PrP formation by unlabeled heterologous PrP-sen. The species of the PrP components (³⁵S-PrP-sen, unlabeled PrP-sen, and PrP-res) in the reactions are indicated by H (hamster), M [mouse or mouse(3F4)], and − (solvent control). −PK and +PK indicate PK-untreated and PK-treated samples, respectively. The molar ratio of ³⁵S-PrP-sen to unlabeled PrP-sen was 1:16. The relative amount of ³⁵S-PrP-sen versus unlabeled PrP-sen was determined by quantitative immunoblot analysis.