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. 2000 May 16;97(11):5836–5841. doi: 10.1073/pnas.110523897

Figure 5.

Figure 5

Dose-dependent binding of 35S-PrP-sen to PrP-res. A total of 20, 40, and 100 kcpm of hamster (Ha) 35S-PrP-sen or the mixture of hamster and mouse(3F4) (Mo) 35S-PrP-sen (see below) were incubated with (solid lines) or without (dashed lines) hamster PrP-res. The binding of 35S-PrP-sen to PrP-res was analyzed by sedimentation analysis as described in the text. The 100-kcpm sample consisted of 20 kcpm of hamster and 80 kcpm of mouse(3F4) 35S-PrP-sen. The 40-kcpm sample consisted of 20 kcpm of hamster and 20 kcpm of mouse(3F4) 35S-PrP-sen. The 20-kcpm sample consisted only of hamster 35S-PrP-sen. The horizontal axis indicates the 35S-PrP-sen added to the reactions (kcpm), whereas the vertical axis indicates 35S-PrP detected in the pelleted fractions. The recoveries of input 35S-PrP varied from 70% to 85%. Each point represents a mean ±SD of triplicates.