Table 2.

GC3 of fugu paralog subcluster protocadherin sequences

Paralog subclusters	GC3 (%)
	5' low homology regiona	3' high homology regiona	p Valueb
FrPcdh2α2–7	41.3 ± 7.2	61.2 ± 0.3	2.78 × 10-4
FrPcdh2α9–25	38.4 ± 4.3	54.4 ± 0.5	2.68 × 10-16
FrPcdh2α26–36	45.0 ± 2.2	60.7 ± 0.7	1.32 × 10-15
FrPcdh2γ1–17	43.6 ± 2.6	56.3 ± 0.4	2.40 × 10-19
FrPcdh2γ19–32'	41.5 ± 3.4	58.5 ± 0.6	3.28 × 10-15
FrPcdh2γ33'-36'	40.1 ± 1.2	68.8 ± 0.3	6.35 × 10-9

^a GC3 is calculated as the average percentage of the GC content at the codon third-position in each paralog subcluster and expressed as mean ± standard deviation.

^b The statistical analysis was conducted using Student's t-test.