Table 1.
Results of Repeated Quantitative Real-time PCR on Cytobrush Specimens
| ID | HPV type assayed | mRNA Log Starting Quantity*** | mRNA Copy Number**** | ||
| Repeat 1 | Repeat 2 | Repeat 1 | Repeat 2 | ||
| 1 | 16 | 1.3845 | 1.409 | 3.99 | 4.09 |
| 2 | 16 | 0 | 0 | 0 | 0 |
| 3 | 16 | 0.7095 | 1.026 | 2.03 | 2.79 |
| 4 | 16 | 0 | 0 | 0 | 0 |
| 5* | 16 | 0 | 0 | 0 | 0 |
| 6 | 16 | 1.5385 | 1.274 | 4.66 | 3.58 |
| 7 | 16 | 1.4075 | 1.4615 | 4.09 | 4.31 |
| 8* | 16 | 0 | 0 | 0 | 0 |
| 9** | 16 | 0 | 0 | 0 | 0 |
| 10 | 16 | 1.5605 | 1.6785 | 4.76 | 5.36 |
| 11 | 16 | 0.737 | 0.8855 | 2.09 | 2.42 |
| 12 | 16 | 0 | 0.253 | 0 | 1.29 |
| 13 | 16 | 0.6945 | 0.656 | 2.00 | 1.93 |
| 14 | 16 | 0.662 | 0.5 | 1.94 | 1.65 |
| 15 | 16 | 1.35 | 1.4165 | 3.86 | 4.12 |
| 16 | 16 | 0.468 | 0.458 | 1.60 | 1.58 |
| 17 | 18 | 0.6625 | 1.0695 | 1.94 | 2.91 |
| 18 | 18 | 4.2175 | 4.1265 | 67.86 | 61.96 |
Note: Coefficient of Variation (CV) = 10.4%
*HPV16 and HPV18 negative, HPV consensus positive; used as specificity control
**HPV16, HPV18, and HPV consensus negative; used as specificity control
***Log SQ = ln(copy number)
****per 20 ng cDNA