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Formation of the predicted peptides. The product pattern of the trimodular NRPS systems was monitored by using Phe, Pro, and the third amino acid in radiolabeled form, TLC separation of the products and subsequent radioactivity scanning. (A) TycA/ProCAT-LeuCATTe with [14C]-Leu. (B) TycA/ProCAT-OrnCAT with [14C]-Orn. (C) TycA/ProCAT-OrnCAT-Te with [14C]-Orn and (D) [14C]-Lys.
