Table 1.

Summary of HIV-1 infection in the DKO-HSC mice

Mouse no.	CD34+ cells	Time after transplantation, wk	HIV (ng/mouse)*	HIV RNA per mL blood (CD4/CD8 ratio)†
				−1 wk after infection	1 wk after infection	2 wk after infection	3 wk after infection	4 wk after infection‡
1	CB	16	NL4-R3A (5)	ND (6.8)	4.0 × 105 (0.6)	2.4 × 104 (0.3)	3.7 × 103§	8.5 × 103§
2	CB	16	NL4-R3A (5)	ND (1.0)	2.0 × 103 (0.5)	2.6 × 104 (0.0)	6.9 × 103§	4.2 × 103§
3	CB	15	NL4-R3A (1)	ND (3.0)	9.7 × 103 (2.9)	9.9 × 104 (1.7)	1.3 × 105 (0.2)	1.9 × 104 (3.7)
4	FL	19	NL4-R3A (1)	ND (1.6)	3.2 × 103 (0.5)	2.5 × 103 (0.8)	2.1 × 103 (0.1)	ND§
5	FL	19	NL4-R3A (1)	ND (1.7)	4.0 × 103 (1.1)	—	—	—
6	FL	41	NL4-R3A (1)	ND (13.5)	1.0 × 105 (1.8)	—	—	—
7	FL	22	JR-CSF (1)	ND (4.0)	1.8 × 104 (5.1)	2.1 × 105 (2.5)	—	—
8	FL	22	JR-CSF (1)	ND (3.6)	2.4 × 105 (3.7)	4.0 × 105 (3.4)	—	—
9	CB	17	NL4-3 (1)	ND (2.7)	7.8 × 104 (0.9)	7.6 × 103 (0.8)	—	8.2 × 104 (0.3)
10	CB	17	NL4-3 (1)	ND (0.9)	ND (1.5)	ND (3.0)	—	—
11	—	—	NL4-R3A (1)	ND§	ND§	ND§	ND§	ND§
12	—	—	NL4-R3A (1)	ND§	ND§	ND§	ND§	ND§
13	CB	16	Mock	ND (1.3)	ND (2.4)	ND (1.7)	ND (3.2)	ND (3.6)
14	CB	16	Mock	ND (1.1)	ND (2.2)	ND (1.7)	—	ND (2.0)

Time after transplantation refers to initiation of HIV-1 infection.

ND indicates nondetectable, below the detection level (< 500 copies/mL); —, not done.

^*The NL4-R3A and NL4 were titered on Hela-CD4-LTR-β-gal cell line. For NL4-R3A, 1 ng p24 equals 4000 infection units. For NL4-3, 1 ng p24 equals 500 infection units. JRCSF was titered on U373-MAGI-CCR5E cells, 1 ng p24 equals 20 infection units. (Both cell lines obtained from NIH AIDS Research and Reference Reagent Program, Division of AIDS, NIAID.)

^†Percentage of CD4⁺ cells divided by percentage of CD8⁺ in the blood.

^‡Mouse no. 3 sample is from 5 weeks after infection.

^§No human CD45⁺ cells detected in the blood.