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. 2007 Feb 9;92(9):3215–3227. doi: 10.1529/biophysj.106.095109

FIGURE 4.

FIGURE 4

Fluorescence-based studies of the interaction of the fluorescein-labeled full-length target operator DNA with CggR. (A) Anisotropy binding titrations of the fluorescein-labeled full DNA operator (OLR) at 0.9 nM with CggR at increasing concentration in absence (▪) or in presence of FBP at 10 μM (▵), 100 μM (•) or 2 mM (⋄); solid line curves correspond to data fitting using the model proposed in Fig. 6 (see the main text). The straight dotted lines indicate the protein concentrations used in Fig. 4 B. (B) FBP titration of CggR in complex with the fluorescein-labeled OLR operator (0.9 nM) at protein concentration of 7.7 nM (♦) and 258 nM (▵) in dimer units. Fittings of the data to models involving one (dashed lines) and two (solid lines) binding sites are shown for comparison. The term mA.U. stands for milli-anisotropy units.